PHYTOCHEMICAL PROFILING of VOLATILE COMPONENTS of Echinacea purpurea (L.) MOENCH MULTIPLIED by MICROPROPAGATION TECHNIQUE

Abstract

Echinacea purpurea (L.) Moench is a substantial herbaceous perennial species of ornamental and medicinal flowering plant that grows to 120 cm in maturity from the Asteraceae family and the Echinacea genus. The development of in vitro protocols for this plant can have a significant impact on the global deployment of this beneficial germplasm. It will also facilitate the rapid multiplication, biotransformation, and genetic transformation of improved phytochemical production, particularly secondary metabolites. All stages of E. purpurea micropropagation in vitro and acclimatization to in vivo conditions were investigated in this study. Furthermore, the obtained volatile components of the plants were determined in this study. The research findings revealed that the best seed sterilization was observed with 15% commercial bleach for 5 minutes. MS plant tissue culture medium including 1.25 mg L^-1 6-benzyl amino purine was suitable for regeneration and the high shoot regeneration was %75 and MS medium containing 1.00 mg L^-1 indole 3 butyric acid was appropriate for rooting. Furthermore, the findings show that peat is an appropriate substance for acclimation of rooted plantlets. Germacrene D, caryophyllene, Borneol, Caryophyllene oxide, and Myrtenal were identified as the main volatile components of E. purpurea by GC/MS and GC/FID analysis.