DEVELOPMENT OF A FAST AND LOW COST METHOD FOR ACETONE-BUTANOL-ETHANOL QUANTIFICATION IN INDUSTRIAL FERMENTATIONS
DOI:
https://doi.org/10.52292/j.laar.2017.297Keywords:
Butanol determination, biofuel, fermentation, industrial media, Butanol determination,, biofuel, fermentation, industrial mediaAbstract
In this work, a method based on mixture density was developed to determine the concentration of acetone-butanol-ethanol solvents (ABE) instead of conventional methods based on gaschromatography. For this purpose, synthetic solutions diluted in distilled water with different concentrations of ABE solvents were used to develop the method. In a second step, different concentrations of ABE solvents were diluted in sweet sorghum juice at different sugar concentration to simulate the real process conditions. The results demonstrated a linear relation between density and ABE concentration in water. For real fermentation media, it was necessary to take into account the concentration of sugar, where the method presented satisfactory results for synthetic and industrial fermentation media. The main contribution of this work was to determine the ABE concentration in a simple, fast and low cost way.
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