ANTIMICROBIAL, ANTIOXIDANT AND CYTOTOXIC EVALUATION OF VARIOUS EXTRACTS OF IFLOGA SPICATA (FORSSK.) SCH.BIP

EXTRACTS OF IFLOGA SPICATA (FORSSK.) SCH.BIP.

Authors

  • Yar KHAN Department of Chemistry, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan
  • JAN Department of Chemistry, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan
  • ULLAH Department of Chemistry, Division of Science and Technology, University of Education Lahore, Punjab, Pakistan
  • AWAN Department of Biotechnology, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan
  • MUHAMMAD Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore, Pakistan
  • Syed Rizvi Hampton University School of Pharmacy

DOI:

https://doi.org/10.52292/j.laar.2021.733

Keywords:

Ifloga spicata, Antimicrobial, Reactive oxygen species, Human blood lymphocytes, Hydrogen peroxide

Abstract

The current study reveals the pharmacological and phytochemical potential of different solvent fractions of Ifloga spicata. Antimicrobial and free radical scavenging activities, DPPH (1, 1-diphenyl-2-picrylhydrazyl), hydrogen peroxide scavenging assay, and cytotoxic effects of plant samples on lymphocytes stressed with hydrogen were performed. Ifloga spicata ethyl acetate fraction markedly showed higher antibacterial potential against P. aeruginosa (ATCC 27853) and E. Coli (ATCC 25922) bacteria. While higher antifungal potential was shown by the chloroform fraction against all three different a strain A. fumigates (ATCC 204305), A. flavus (ATCC 9643) and A. niger (ATCC 16404) as compared to others fractions of sample. DPPH maximum antioxidant potential was noted for methanolic extract followed by ethyl acetate and chloroform. Similarly, hydrogen peroxide effect was significantly decreased by ethyl acetate fraction. In blood lymphocytes the raise in ROS and TBARS level due to stress given by H2O2 was also recovered by different extracts of I. spicata. The decrease level of CAT, SOD and POD was also restored

Published

2021-09-30