Interactions between alginate and gelatin in biosensor active layer design: SEM, FTIR and electrochemical analyzes
DOI:
https://doi.org/10.52292/j.laar.2026.1094Keywords:
Hydrogel, Polymer, Peroxidase, Glucose oxidaseAbstract
In electrochemical biosensors, all efforts are focused on its immobilization between the biocomponent and the analyte. This preserves biological activity and keeps the biocomponent close to the electrode. The aim of this study is to test the efficacy of the designed alginate-gelatin based polymers for use in biosensors. SEM and FTIR analyzes of alginate and alginate-gelatin polymer were investigated. EDC: NHS was used as crosslinking agent. The Glucose Oxidase-Peroxidase enzyme system was used. In SEM analysis, changes occurred in the supramolecular structure. It is thought that the polymer has a porous foam-like appearance and the cavities it contains will act as a basis for enzyme activity. In the FTIR spectra, we report those strong intermolecular interactions occur between alginate-gelatin and EDC: NHS. Redox peaks were found in the range of 0-1.2 V in cyclic voltammograms. Our results show that alginate-gelatin-EDC: NHS polymer structure can be used effectively in active layer design.
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