EVALUATION OF BIOHYDROGEN PRODUCTION FROM SUGARCANE VINASSE IN AN ANAEROBIC FLUIDIZED BED REACTOR WITHOUT PH CONTROL

  • Georgia Gois Federal University of Alagoas
  • Williane Macêdo University of São Paulo
  • Fernanda Santana Peiter
  • Taciana Chaves Federal University of Alagoas
  • Vinícius Sales Federal University of Alagoas
  • Pablo Barbosa Federal University of Alagoas
  • Ana Abud Federal University of Sergipe
  • Eduardo Lucena Cavalcante Amorim
Keywords: Sugarcane vinasse, Biohydrogen production, Anaerobic fluidized bed reactor, No-control pH

Abstract

This study aimed to produce biohydrogen from sugarcane vinasse in an anaerobic fluidized bed reactor operated at ambient temperature (26-35 °C) with a progressive increase of the organic load rate (OLR) (17-155 kg-COD m-3 d-1) and without influent pH control. Hydraulic retention times (HRT) of 12, 6 and 4 h were applied to increase the OLR. The highest hydrogen yield, hydrogen production rate and percentage of hydrogen in the biogas were obtained in the HRT of 4 h, corresponding to the values of 2.40 mol-H2 mol-1-carbohydrate, 0.33 L-H2 h-1 L-1-reactor, and 33%, respectively. The butyric acid fermentative route was favored in the process. The PCR/DGGE analysis showed that the microbial community structure was modified along operational stages and increasing OLR caused an increase in microbial diversity. The results indicate that biohydrogen production from anaerobic digestion of sugarcane vinasse can be achieved with no alkalization pretreatment.

Published
2020-12-24