HIGH-SENSITIVE BIOACTIVITY ASSAY FOR HYBRID NANOSTRUCTURED MATERIALS WITH PLASMONIC PROPERTIES

Authors

  • J. L. HERNÁNDEZ-LÓPEZ

DOI:

https://doi.org/10.52292/j.laar.2014.428

Keywords:

Au-NRs/Neutravidin, LSPR, enzyme activity, in-vivo imaging, biological sensing

Abstract

The binding of biotin to gold nanorods (Au-NRs) conjugated to neutravidin is accompanied by an increase in the fluorescence intensity of protein´s tryptophan residues. Localized surface plasmon resonance (LSPR), combined with the known affinity of streptavidin for biotin, led to the development of a high-sensitive enzyme activity assay.

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Published

2014-04-30

Issue

Vol. 44 No. 2 (2014): Latin American Applied Research

Section

Applied Chemistry

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